Methods

Rapid and efficient induction of functional astrocytes from human pluripotent stem cells Rapid and efficient induction of functional astrocytes from human pluripotent stem cells, Published online: 20 August 2018; doi:10.1038/s41592-018-0103-2 A simple transcription-factor-based protocol generates functional astrocytes rapidly and efficiently from human pluripotent stem cells.
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Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells, Published online: 20 August 2018; doi:10.1038/s41592-018-0104-1 This paper describes modifications to standard culture conditions that permit the growth of naive human pluripotent stem cells with reduced genomic instability.
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Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging, Published online: 20 August 2018; doi:10.1038/s41592-018-0105-0 Slow off-rate modified aptamer (SOMAmer) reagents are small and versatile probes for DNA-PAINT super-resolution microscopy that enable multiplexed, quantitative, and high-resolution imaging in fixed and live cells.
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The GAGOme: a cell-based library of displayed glycosaminoglycans The GAGOme: a cell-based library of displayed glycosaminoglycans, Published online: 13 August 2018; doi:10.1038/s41592-018-0086-z A CHO cell library displaying a near-complete repertoire of glycosaminoglycan (GAG) modifications provides a resource for cell-based binding assays, recombinant proteoglycan expression, and assembly of GAG glycan microarrays.
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Generation and post-injury integration of human spinal cord neural stem cells Generation and post-injury integration of human spinal cord neural stem cells, Published online: 06 August 2018; doi:10.1038/s41592-018-0074-3 Spinal cord neural stem cells are generated from human pluripotent stem cells via a chemically defined, xeno-free method, and exhibit efficient and functional engraftment in rat spinal
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Water content, not stiffness, dominates Brillouin spectroscopy measurements in hydrated materials Water content, not stiffness, dominates Brillouin spectroscopy measurements in hydrated materials, Published online: 31 July 2018; doi:10.1038/s41592-018-0076-1 Water content, not stiffness, dominates Brillouin spectroscopy measurements in hydrated materials
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Quanti.us: a tool for rapid, flexible, crowd-based annotation of images Quanti.us: a tool for rapid, flexible, crowd-based annotation of images, Published online: 31 July 2018; doi:10.1038/s41592-018-0069-0 Annotated image data are required for image analysis, to test analytical methods, and to train learning algorithms. This paper describes and characterizes a tool that allows researchers to crowdsource
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Finally: development of humanized lymph nodes Finally: development of humanized lymph nodes, Published online: 31 July 2018; doi:10.1038/s41592-018-0080-5 Overexpression of mouse thymic-stromal-cell-derived lymphopoietin in immune-compromised mice that harbor a reconstituted human immune system rescues lymph node formation and enhances adaptive immune responses.
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Artifact-free high-density localization microscopy analysis Artifact-free high-density localization microscopy analysis, Published online: 30 July 2018; doi:10.1038/s41592-018-0072-5 Preprocessing of localization microscopy datasets using Haar wavelet kernel (HAWK) analysis enables artifact-free analysis of high-density data for improved fixed and live-cell super-resolution microscopy.
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Induction of myelinating oligodendrocytes in human cortical spheroids Induction of myelinating oligodendrocytes in human cortical spheroids, Published online: 25 July 2018; doi:10.1038/s41592-018-0081-4 A method for generating cortical spheroids from human pluripotent stem cells produces maturing oligodendrocytes that can myelinate axons and model myelin disease and drug effects.
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High-precision automated reconstruction of neurons with flood-filling networks High-precision automated reconstruction of neurons with flood-filling networks, Published online: 16 July 2018; doi:10.1038/s41592-018-0049-4 Flood-filling networks are a deep-learning-based pipeline for reconstruction of neurons from electron microscopy datasets. The approach results in exceptionally low error rates, thereby reducing the need for extensive human proofreading.
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Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections, Published online: 16 July 2018; doi:10.1038/s41592-018-0053-8 Active PSF shaping and adaptive optics are combined to enable 3D localization microscopy throughout thick tissues. The method was used to study the
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A synthetic-diploid benchmark for accurate variant-calling evaluation A synthetic-diploid benchmark for accurate variant-calling evaluation, Published online: 16 July 2018; doi:10.1038/s41592-018-0054-7 The synthetic-diploid (Syndip) benchmark dataset, constructed from two fully homozygous long-read assemblies, provides more accurate assessments of error rates in small-variant-calling algorithms than existing benchmarks.
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An enhanced CRISPR repressor for targeted mammalian gene regulation An enhanced CRISPR repressor for targeted mammalian gene regulation, Published online: 16 July 2018; doi:10.1038/s41592-018-0048-5 The fusion of dead Cas9 with KRAB and the transcriptional repressor domain of the chromatin modifier MeCP2 leads to an efficient transcriptional silencer that can be applied to genome-scale screens and
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Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy, Published online: 09 July 2018; doi:10.1038/s41592-018-0052-9 Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the
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