Guide Swap enables genome-scale pooled CRISPR–Cas9 screening in human primary cells

Methods

Guide Swap enables genome-scale pooled CRISPR–Cas9 screening in human primary cells

Guide Swap enables genome-scale pooled CRISPR–Cas9 screening in human primary cells, Published online: 08 October 2018; doi:10.1038/s41592-018-0149-1

Guide Swap challenges the hypothesis that Cas9–sgRNA binding is irreversible. The authors find that instead, nontargeting sgRNAs are swapped for targeting sgRNAs in the Cas9 complex. The method allows genome-scale functional screens in primary cells

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