Nearly a century after the film “Reefer Madness” alarmed the nation, some policymakers and doctors are again becoming concerned about the dangers of marijuana, although the reefers are long gone. Experts now distinguish between the “new cannabis” — legal, highly potent, available in tabs, edibles and vapes — and the old version, a far milder
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Can you see me?Marcel van Kammen/Minden By Michael Marshall Peacock tails are brightly coloured and highly conspicuous – but maybe not to their predators. The animals that hunt peacocks have poor colour vision and, to their eyes, the birds’ tails may actually help them blend into the background. However, it is not clear whether this colour-based
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Posted by: RNA-Seq Blog in Webinars 4 hours ago 105 Views 28 Feb 2019 at 11:00 (GMT -06:00) Jonathan Göke – Senior Research Scientist (PI) Genome Institute of Singapore (A*STAR) The ability of RNA-sequencing to generate a high-dimensional, quantitative readout of cells and tissues has made it arguably the most successful functional genomics assay in
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Description Our lab is interested in transcriptome and epigenetic changes that occur in a time-dependent manner during pregnancy and how those can influence autoimmune disease. We are seeking a highly motivated and creative postdoctoral fellow to examine changes in coding and non-coding RNA patterns over time (based on RNA-seq data) in the context of human
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Share to facebook Share to twitter Share to linkedin What are the risks of radiotherapy? originally appeared on Quora: the place to gain and share knowledge, empowering people to learn from others and better understand the world. Answer by Gary Larson, Board Certified Radiation Oncologist, on Quora: There are multiple potential risks of Radiation Therapy
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Overview In this work, we present an experimental and analytical framework where we systematically assay and profile DNA damage, excision repair, and gene expression in a genome-wide fashion across four mouse organs. Figure 1a outlines the experimental design. Specifically, cisplatin was administered by an intraperitoneal injection in mice. DNA damage, excision repair, and gene expression were
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Fluorescence microscopy is the method of choice to visualize specific cellular organelles, proteins, or nucleic acids with high sensitivity and selectivity. Importantly, fluorescence is, in principle, quantitative in that intensity of fluorescence from each position in a sample is proportional to the abundance of the fluorescent moiety in that region of the sample. Once fluorescence
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Task 1: Discriminating pathogenicity of alternative alleles Given a genomic position with segregating alleles in a population, except in rare instances of balancing selection, one allele will usually be more deleterious than the other, presumably contributing to pathogenesis30,31,32,33. At any position, apart from the reference allele, we need to know which of the other three
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Genome-wide association studies (GWAS) We first performed standard GWAS of 21 traits in the Hutterites (Supplementary Table 1). These studies identified one genome-wide significant association (p < 5 × 10–8) with each of five of the 21 traits: low-density lipoprotein level (LDL)-cholesterol, triglycerides, carotid artery intima media thickness (CIMT), left ventricular mass index (LVMI), and monocyte count. The results of
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Next generation sequencing (NGS) [1] technologies greatly promote research in genome-wide mRNA expression data. Compared with microarray technologies, NGS provides higher resolution data and more precise measurement of levels of transcripts for studying gene expression. Through downstream analysis of RNA sequencing (RNAseq) data, gene expression levels reveal the variability between different samples. Typically, in RNAseq
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Primer-dimer prediction To begin development on our dimer prediction algorithm PrimerDimer, a set of primer-dimer artefacts were first sequenced to assess the types of primer-primer interactions that cause dimer formation (Supplementary Fig. S1). These sequences confirm what other studies have previously stipulated—that the primer-primer interactions of most concern contain stable complements at the 3′ ends (Fig. 1A),
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1. Visscher PM, Wray NR, Zhang Q, et al. 10 years of GWAS discovery: biology, function, and translation. Am J Hum Genet. 2017;101:5–22. 2. Stranger BE, Stahl EA, Raj T. Progress and promise of genome-wide association studies for human complex trait genetics. Genetics. 2011;187:367–83. 3. Price AL, Spencer CCA, Donnelly P. Progress and promise in
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1. Ungaro, R., Mehandru, S., Allen, P. B., Peyrin-Biroulet, L. & Colombel, J.-F. Ulcerative colitis. Lancet 389, 1756–1770 (2017). 2. Torres, J., Mehandru, S., Colombel, J.-F. & Peyrin-Biroulet, L. Crohn’s disease. Lancet 389, 1741–1755 (2016). 3. de Souza, H. S. P., Fiocchi, C. & Iliopoulos, D. The IBD interactome: an integrated view of aetiology, pathogenesis
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Analysis of HIV replication dynamics SupT1 CD4+ T cells were either Mock-infected or infected with an HIVeGFP-based vector. Virus-host interactions were characterized over time by multiple time series measurements (Fig. 1). As described previously18, the infection conditions allowed for quasi universal infection. Through an initial quantitative analysis, levels of viral intermediates – early and late reverse
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Enlarge this image A so-called “blood moon” seen during a total lunar eclipse in western Germany, on Sept. 28, 2015. Patrik Stollarz/AFP/Getty Images hide caption toggle caption Patrik Stollarz/AFP/Getty Images Updated at 9:50 p.m. ET A special celestial event is on the calendar for this Sunday night and experts are already raving: “A full 62
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Abstract We investigate how the neural processing in auditory cortex is shaped by the statistics of natural sounds. Hypothesising that auditory cortex (A1) represents the structural primitives out of which sounds are composed, we employ a statistical model to extract such components. The input to the model are cochleagrams which approximate the non-linear transformations a
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Abstract Complex eukaryotic promoters normally contain multiple cis-regulatory sequences for different transcription factors (TFs). The binding patterns of the TFs to these sites, as well as the way the TFs interact with each other and with the RNA polymerase (RNAp), lead to combinatorial problems rarely understood in detail, especially under varying epigenetic conditions. The aim
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Citation: Erickson KE, Rukhlenko OS, Shahinuzzaman M, Slavkova KP, Lin YT, Suderman R, et al. (2019) Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor. PLoS Comput Biol 15(1): e1006706. https://doi.org/10.1371/journal.pcbi.1006706 Editor: Anand R. Asthagiri, Northeastern University, UNITED STATES Received: March 21, 2018; Accepted: December 9, 2018; Published: January 17,
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Next, we compared the behaviour of the fitted models to the behaviour of the ‘true’ parameters, to determine the information in each dataset on The effect of each immune component in controlling a single infection. In Fig 3, we removed various immune components from the model. We then compared predictions of the viral load for
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Enlarge this image President Trump called for a beefing up of existing defenses, such as the Aegis ashore system pictured. In addition, he called for research into new advanced concepts. Mark Wright/Missile Defense Agency hide caption toggle caption Mark Wright/Missile Defense Agency Updated at 5:47 p.m. ET President Trump unveiled a sweeping plan Thursday to
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WASHINGTON (Reuters) – Saturn’s rings are one of our solar system’s magnificent sights, but may be a relatively recent addition, according to data obtained from NASA’s Cassini spacecraft before the robotic explorer’s 2017 death plunge into the giant gas planet. FILE PHOTO: These Hubble Space Telescope images of Saturn, shown in this handout composite photo released
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Global leaders are increasingly worried about environmental issuesJustin Sullivan/Getty By Graham Lawton The top five dangers facing the world are all now either environmental or technological, according to a major annual survey. The World Economic Forum’s 2019 Global Risks Report has found that extreme weather, climate policy failure and natural disasters are the risks most
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Jan 17 2019 Company recognized for its strategic use of social media platforms Merck, a leading science and technology company, today announced that it has been recognized by BioInformatics LLC with a 2018 Life Science Industry Award® for best use of social media. “This recognition reflects the opinions of our scientific customers and industry peers,”
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Posted by: RNA-Seq Blog in Analysis Pipelines, Annotation 25 mins ago 37 Views Circular RNAs are an emerging class of transcript isoforms created by unique back splicing of exons to form a closed covalent circular structure. While initially considered as product of aberrant splicing, recent evidence suggests unique functions and conservation across evolution. While circular
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