Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy

Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy

Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy, Published online: 17 September 2018; doi:10.1038/s41592-018-0111-2

Convolutional neural networks enable prediction of fluorescently labeled structures from three-dimensional time-lapse transmitted-light images. Applications include multiplexed long time-lapse imaging and prediction of fluorescence in electron micrographs.

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